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KMID : 0387820100170010051
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Clinical Pediatric Hematology-Oncology
2010 Volume.17 No. 1 p.51 ~ p.58
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Hypoxic Treated Bone Marrow Cells Enhanced Foxp3 and IL-10 Expression in a Murine Liver Injury Model
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Joo Sun-Young
Jung Sun-Ok
Cho Kyung-Ah
Woo So-Youn
Seoh Ju-Young
Ryu Kyung-Ha
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Abstract
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Purpose: It has been reported that bone marrow (BM)-derived stem cells retain the capability of regenerating organs, as well as functioning as an immune modulator. We previously reported that liver function recovered with bone marrow cells (BMCs) transplantation in a liver failure mouse by CCl4 injection and after hypoxic conditioned BMCs transplantation, cell migration and engraftment had been improved. We investigated immune modulation after hypoxic conditioned BMCs transplantation.
Methods: To induce the acute liver failure, C57BL/6 mice were injected intra-peritoneally once a day with CCl4 (10microliter/g) for two consecutive days, and were used as liver injury recipients. Donor BMCs were incubated under the desired level of O2 (0.1% and 20%) for 24 hours. We then injected the conditioned BMCs through the tail vein into the CCl4-injected mice. For further analyzing immunological changes after transferring BMCexposed to hypoxia to CCl4 treated mice, we separated spleen cells after BMCs transplantation and analyzed phenotype changes of Foxp3, IL-10, IL-4, IL-12 and IFN- of CD4+ T cells.
Results: The proportion of CD4+CD25+Foxp3+ cells, IL-10 secreting cells increased in CCl4 damaged mice after the BMCs transfers after hypoxic treatment. Although the proportion of IL-4 expressing CD4+ cells increased under the hypoxic conditions, did not have statistical significance. The percentage IFN-gamma and IL-12 expressing CD4+ cells did not show statistical significance.
Conclusion: It is suggested that hypoxic conditioned BMCs transplantation affected the immune modulation via inducing Foxp3+ and IL-10 secreting CD4+ cells in CCl4 damaged mice.
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KEYWORD
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Carbon tetrachloride, Bone marrow cells, Hypoxia, Regulatory T cell, Foxp3
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